The host immunologic response to group A streptococcal infections gives rise to numerous
antibodies directed against cellular and extracellular bacterial antigens. For determining
individual immune status, or studying the pathogenesis of group A streptococcal associated
diseases, such as acute rheumatic fever (ARF), an assay capable of determining antibodies
responses to multiple antigens would be of great advantage. We have developed a
microsphere based, multiplexed immunoassay for the simultaneous quantitation of …

Neisseria meningitidis is a gram-negative bacterium causing disease world wide with a
fatality rate of 5–10%. Five serogroups, A, B, C, Y and W-135 are responsible for virtually all
cases of the disease in humans. We have developed a multiplexed assay for the
simultaneous quantitation of IgG antibody responses to the four most immunogenic (A, C, Y,
and W-135) N. meningitidis serogroups. A simple and less manipulative method was
employed for conjugation of the capsular polysaccharide antigens to the microspheres. The …